Anti-DNLZ Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q5SXM8

Core Product Specifications and Parameters

Biological Background: DNLZ Function and Localization

• DNLZ, or DNL-type zinc finger protein, is also known as Hsp70-escort protein 1 (HEP1) and is a co-chaperone for mitochondrial HSPA9/mortalin, helping to prevent its self-aggregation.
• The protein localizes to the mitochondrion, where it assists in mitochondrial protein quality control and import processes.
• DNLZ contains a zinc finger domain and requires metal binding for its structural integrity and function.
• It possesses a transit peptide for mitochondrial targeting, indicating post-translational import into the organelle.
• As a reference proteome component, DNLZ is conserved and studied across species, underscoring its fundamental role in cellular proteostasis.
• The gene DNLZ (formerly C9orf151) is located on chromosome 9 in humans.
• Its molecular weight is approximately 19 kDa, consistent with a small co-chaperone.

Experimental Guidance and Technical Tips

• The immunogen used is recombinant full-length human DNLZ protein, suggesting the antibody may recognize native and denatured forms. For WB, consider loading 20-30 µg of mitochondrial-enriched lysate and using appropriate blocking.
• In IF/ICC experiments, co-staining with mitochondrial markers (e.g., MitoTracker or anti-TOM20) is recommended to confirm mitochondrial localization.
• For ELISA, the antibody can be explored as both capture and detection reagent, with optimization of coating concentrations.
• Since DNLZ functions as a co-chaperone, experimental conditions that induce mitochondrial stress may enhance detection sensitivity.
• Always validate the antibody in your specific sample system, as cross-reactivity and performance can vary with tissue and cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials, offering a comprehensive portfolio from validated antibody pairs to bulk ancillary reagents. Focusing on mitochondrial biology and chaperone targets like DNLZ, we provide reliable access to monoclonal and polyclonal antibodies, recombinant proteins, and assay development tools. Whether you need standard products or rare target sourcing, our one-stop supply chain accelerates your diagnostic and research workflows from concept to clinic.