Anti-DNER Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NFT8

Core Product Specifications and Parameters

Biological Background: DNER Function and Localization

• DNER (Delta and Notch-like epidermal growth factor-related receptor) is also known as bet and UNQ26; it is a single-pass transmembrane protein.
• It functions as an activator of the NOTCH1 signaling pathway, potentially mediating neuron-glia interactions during astrocytogenesis.
• The protein contains multiple EGF-like domains and belongs to the Delta/Serrate/Lag2 (DSL) family of Notch ligands.
• DNER is localized to the cell membrane.
• It is heavily glycosylated and contains disulfide bonds that stabilize its EGF-like repeats; phosphorylation modifications have been observed.
• Expression is predominantly in the brain, spinal cord, and adrenal gland.
• The calculated molecular weight is approximately 78.5 kDa, consistent with the observed 78 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 140-240) of human DNER, which may contribute to specificity for the mature protein.
• For western blot (WB), consider a starting dilution of 1:500-1:2000 and validate with appropriate positive (e.g., brain lysate) and negative controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization conditions should be optimized for membrane-bound targets.
• ELISA can be used for quantitative detection of DNER; pair with a matched detection antibody if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with one-stop access to IVD raw materials, including validated antibody pairs and bulk reagents. For Notch signaling and neurological studies, we offer this DNER polyclonal antibody alongside a range of matched ancillary reagents. Contact us for custom sourcing of rare target raw materials to accelerate your assay development.