Anti-DNASE1 Rabbit Polyclonal Antibody for WB, ELISA - P24855

Core Product Specifications and Parameters

Biological Background: Deoxyribonuclease-1 (DNASE1) Function and Localization

• Encoded by the DNASE1 gene (also known as DNL1, DRNI), deoxyribonuclease-1 (DNase I) is a secreted endonuclease that preferentially cleaves protein-free DNA. Related references: PMID:11241278 PMID:2251263 PMID:2277032
• Plays a role in apoptosis by mediating DNA fragmentation during programmed cell death. Related references: PMID:11241278
• Binds specifically to G-actin and blocks actin polymerization, linking nuclease activity to cytoskeletal regulation.
• Together with DNASE1L3, degrades neutrophil extracellular traps (NETs) to prevent clot formation and organ damage during inflammation.
• Contains a signal peptide for secretion, and is found in secreted form, zymogen granules, and the nuclear envelope.
• Principally expressed in tissues of the digestive system, with highest levels in urine, semen, and saliva.
• Features include calcium-dependent activity, disulfide bonds, glycosylation, and potential alternative splicing isoforms.
• Clinically relevant in systemic lupus erythematosus (SLE) due to its role in clearing circulating DNA.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 22-282 of human DNASE1, covering the majority of the mature protein; this design likely recognizes native and denatured forms, making it suitable for Western blot (31 kDa) and ELISA applications.
• For Western blot, consider using reducing conditions and appropriate tissue or cell lysates from human, mouse, or rat samples; validate loading controls.
• In ELISA, pair with a matched detection antibody or use as a capture/detection reagent; optimize coating and blocking conditions.
• Since DNASE1 is secreted, serum, urine, or cell supernatant may be relevant samples; consider additives to stabilize the enzyme if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies, antigen pairs, and bulk reagents to support your DNASE1 research. Our supply network covers nucleases, apoptosis, and NET-related targets, with expertise in bridging from concept to clinic.