Anti-DNAL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q4LDG9

Core Product Specifications and Parameters

Biological Background: DNAL1 Function and Localization

• DNAL1 encodes dynein axonemal light chain 1, an essential component of the outer arm dynein (ODA) multisubunit ATPase complex in motile cilia.
• It plays a critical role in generating force for ciliary motility and governing beat frequency (By similarity).
• DNAL1 is involved in a mechanosensory feedback mechanism that controls ODA activity by tethering the outer arm dynein heavy chain (DNAH5) to the microtubule within the axoneme (By similarity).
• This protein is important for ciliary function in the airways and for the cilia that produce nodal flow essential for left-right asymmetry determination (PubMed:21496787). Related references: PMID:21496787
• DNAL1 localizes to the cytoplasm, specifically to the cilium axoneme cytoskeleton.
• It is expressed in tissues carrying motile cilia, such as respiratory epithelia, ependyma, and testis.
• Mutations in DNAL1 are associated with ciliopathies, including primary ciliary dyskinesia (CILD16) and Kartagener syndrome.
• The protein contains leucine-rich repeats and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• Raised against a recombinant fragment (aa 41-190) of human DNAL1; verify epitope recognition if precise mapping is required.
• For Western blot, a band around 22 kDa is expected; consider using reducing conditions and validate with positive controls such as testis or respiratory epithelial lysates.
• In immunofluorescence/ICC, target the cilium axoneme; co-stain with an axoneme marker to confirm specificity.
• For ELISA, the antibody may be suitable as capture or detection reagent; perform checkerboard titrations for optimal pairing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents for ciliary biology and respiratory disease research. Whether you need this anti-DNAL1 polyclonal antibody or custom rare-target raw materials, our sourcing platform streamlines your IVD development from concept to clinic.