Anti-DNAL1 Rabbit Polyclonal Antibody for WB and ELISA - Q4LDG9

Core Product Specifications and Parameters

Biological Background: DNAL1 Function and Localization

• Encodes dynein axonemal light chain 1 (DNAL1), a component of the outer arm dynein (ODA) in motile cilia.
• Part of multisubunit axonemal ATPase complexes that generate force for cilia motility and regulate beat frequency (By similarity).
• Functions in a mechanosensory feedback mechanism tethering ODA heavy chain DNAH5 to microtubules, modulating ODA activity in response to conformational cues (By similarity).
• Critical for ciliary function in airway epithelia and for generating nodal flow essential for left-right body axis determination. Related references: PMID:21496787
• Localizes to the axoneme of motile cilia within the cytoplasm and cytoskeleton.
• Expressed specifically in tissues with motile cilia, including respiratory epithelia, ependyma, and testis.
• Post-translationally modified by acetylation and phosphorylation; associated with primary ciliary dyskinesia and Kartagener syndrome.
• Contains leucine-rich repeats (LRR) that may facilitate protein-protein interactions within the axonemal dynein complex.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 41–190 of human DNAL1, a region that may be conserved across species, potentially contributing to the observed cross-reactivity with mouse and rat.
• For Western blot, a band around 22 kDa is expected; consider using appropriate protein markers and optimizing SDS-PAGE conditions for low molecular weight targets.
• In ELISA applications, the recombinant protein used for immunization could serve as a positive control; assess antibody titer and specificity in your sample matrix.
• For ciliary protein studies, sample preparation may require specialized extraction methods to enrich axonemal fractions; validate antibody performance in the relevant tissue or cell system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials for ciliary biology and respiratory disease research, including validated antibody pairs, optimized polyclonal antibodies like this anti-DNAL1 reagent, and bulk ancillary reagents. Our sourcing expertise covers rare targets and specialized raw materials, supporting your assay development from concept to clinic.