Anti-DNAJC9 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WXX5

Core Product Specifications and Parameters

Biological Background: DNAJC9 Function and Localization

• DNAJC9, also known as DnaJ homolog subfamily C member 9 or DnaJ protein SB73, is a 30 kDa protein belonging to the DnaJ/Hsp40 family, sharing structural features with J-domain co-chaperones.
• It functions as a dual histone chaperone and heat shock co-chaperone, interacting with histone H3-H4 heterodimers and assisting MCM2 in nucleosome assembly. Related references: PMID:33857403
• DNAJC9 also acts as a co-chaperone for the HSP70 family, including HSPA1A, HSPA1B, and HSPA8, recruiting them to histone substrates to maintain chromatin integrity. Related references: PMID:17182002, PMID:33857403
• It forms a co-chaperone complex with TONSL and recruits histone chaperones ASF1A, NASP, and SPT2, facilitating histone H3-H4 transfer and chromatin assembly. Related references: PMID:33857403
• Subcellularly, DNAJC9 localizes to the nucleus, cytoplasm, and cell membrane, consistent with its roles in chromatin dynamics and heat shock responses.
• The protein is subject to phosphorylation and has been identified in multiple proteomics studies, indicating regulation by post-translational modification.
• DNAJC9 is broadly expressed across tissues including heart, placenta, liver, skeletal muscle, kidney, pancreas, thymus, ovary, colon, and peripheral blood.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human DNAJC9 protein (aa 1–260), which may provide broad epitope recognition and high sensitivity. Optimize antibody dilution for each application.
• For WB, the expected band is ~30 kDa; include appropriate positive control lysates from human cell lines expressing DNAJC9.
• In IF/ICC, consider fixation/permeabilization conditions that preserve nuclear and cytoplasmic epitopes, and validate with DNA or organelle markers.
• Cross-reactivity is reported for human; if working with other species, verify sequence homology and perform validation experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets such as DNAJC9—involved in chromatin biology and protein quality control—we offer reliable access to niche raw materials and can assist with rare target sourcing to accelerate your assay development and clinical translation projects.