Anti-DNAJC7 Polyclonal Antibody for WB, ELISA - Q99615

Core Product Specifications and Parameters

Biological Background: DNAJC7 Function and Localization

• DNAJC7, also known as TPR repeat protein 2 (TPR2), is a member of the DnaJ homolog subfamily C. It contains tetratricopeptide repeat (TPR) domains critical for protein-protein interactions.
• Functions as a co-chaperone that regulates HSP70 and HSP90 molecular chaperones, particularly in the folding and maturation of steroid hormone receptors like glucocorticoid and progesterone receptors.
• Proposed to act as a recycling chaperone, facilitating the return of incompletely folded substrates to early chaperoning stages. In vitro, it induces ATP-independent dissociation of HSP90 from chaperone-substrate complexes.
• Recruits the nuclear receptor NR1I3 to the cytoplasm, influencing its subcellular localization and activity.
• Localizes broadly in the cytoplasm, nucleus, and associates with the cytoskeleton, suggesting dynamic roles in protein folding, trafficking, and signaling.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its chaperone activity and interactions.
• Alternative splicing gives rise to multiple isoforms, potentially fine-tuning chaperone functions in different tissues or conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 230-380) of human DNAJC7; consider verifying specificity using appropriate controls and knockdown or knockout models.
• For Western blot, a predominant band near 56 kDa is expected. However, multiple isoforms and post-translational modifications may cause additional bands or slight migration shifts.
• This antibody is validated for ELISA; when setting up sandwich assays, pair with a carefully titered detection antibody to achieve optimal sensitivity.
• Cross-reactivity is reported for human; if using with samples from other species, sequence homology of the immunogen region should be examined and reactivity validated empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your development workflow by providing a comprehensive range of IVD raw materials including validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For researchers focused on co-chaperone biology and steroid receptor signaling, CamelBio offers reliable access to well-characterized DNAJC7 antibodies and supports seamless transition from concept to clinical application.