Anti-DNAJC5 Polyclonal Antibody for WB and ELISA - Q9H3Z4

Core Product Specifications and Parameters

Biological Background: DNAJC5 Function and Localization

• DNAJC5, also known as cysteine string protein (CSP) or ceroid-lipofuscinosis neuronal protein 4 (CLN4), is a member of the DnaJ/Hsp40 chaperone family.
• Acts as a general chaperone in regulated exocytosis and as a co-chaperone for the SNARE protein SNAP-25.
• Involved in calcium-mediated control of a late stage of exocytosis and may be important for presynaptic function and calcium-dependent neurotransmitter release.
• Localizes to the cytoplasm, cell membrane, and cytoplasmic vesicles including chromaffin granule membranes and melanosomes.
• Expressed in a wide range of tissues: pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, and heart.
• Undergoes post-translational modifications such as palmitoylation, phosphorylation, and acetylation.
• Mutations in DNAJC5 are associated with neuronal ceroid lipofuscinosis (CLN4 disease), a neurodegenerative disorder.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-198), which may detect full-length DNAJC5 and potential isoforms.
• For WB, a band around 22 kDa is expected; validate in relevant tissue/cell lysates expressing DNAJC5 (e.g., brain, pancreas).
• Cross-reactivity with mouse and rat allows use in rodent models; verify performance in the appropriate species.
• For ELISA, consider using the recombinant protein as a standard or coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for neuroscience and neurodegeneration research. We also offer rare target raw-material sourcing to accelerate your assay development.