Anti-DNAJC2 Polyclonal Antibody for WB, ELISA - Q99543

Core Product Specifications and Parameters

Biological Background: DNAJC2 Function and Localization

• DNAJC2 (DnaJ homolog subfamily C member 2), also known as ZRF1, MPP11, or MPHOSPH11, is a member of the DNAJ/HSP40 family of co-chaperones.
• It acts as a molecular chaperone in the cytosol as part of the ribosome-associated complex (RAC), where it stimulates the ATPase activity of Hsp70-type chaperones (HSPA14) to assist in folding nascent polypeptides.
• In the nucleus, DNAJC2 functions as a chromatin regulator by recognizing histone H2A ubiquitinated at Lys-119 (H2AK119ub) and promoting the displacement of polycomb PRC1 complex, thereby facilitating transcription activation.
• The protein localizes to both the nucleus and the cytosol.
• DNAJC2 is widely expressed across tissues.
• Post-translational modifications include acetylation and phosphorylation; it is also identified as a phosphoprotein.
• The molecular weight is approximately 72 kDa (calculated 71996 Da), consistent with the protein weight of the antibody product.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-140) of human DNAJC2, which may target the conserved J-domain; verify specificity in the relevant species.
• For Western blot (WB), a band at approximately 72 kDa is expected; consider optimizing antibody dilution and using appropriate loading controls.
• This antibody cross-reacts with mouse DNAJC2, making it potentially suitable for studies in mouse models; validate performance in mouse-derived samples.
• For ELISA, coat with recombinant DNAJC2 protein or use it for capture/detection; titration and blocking steps should be optimized empirically.
• Since DNAJC2 has both cytoplasmic and nuclear functions, subcellular fractionation may aid in detecting localization-specific forms.

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CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Our portfolio includes optimized polyclonal antibodies like this anti-DNAJC2 antibody for applications in chaperone biology and chromatin regulation studies. For rare targets or custom sourcing, CamelBio acts as your dedicated procurement bridge to accelerate development from concept to clinic.