Anti-DNAJC19 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96DA6

Core Product Specifications and Parameters

Biological Background: DNAJC19 Function and Localization

• DNAJC19, also known as Mitochondrial import inner membrane translocase subunit TIM14 or DnaJ homolog subfamily C member 19, is a 12 kDa protein encoded by the DNAJC19 (TIM14, TIMM14) gene in humans.
• It functions as a mitochondrial co-chaperone that forms a complex with prohibitins to regulate cardiolipin remodeling.
• DNAJC19 is a component of the PAM complex, which is required for the ATP-dependent translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix.
• The protein localizes to the mitochondrion inner membrane and is ubiquitously expressed in human tissues.
• Keywords associated with DNAJC19 include Acetylation, Alternative splicing, Cardiomyopathy, Chaperone, Membrane, Mitochondrion, Mitochondrion inner membrane, Phosphoprotein, Protein transport, Translocation, Transmembrane, and Transport.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to amino acids 1-116 of human DNAJC19, covering the N-terminal region. It may recognize full-length protein and potential isoforms or cleavage products.
• For Western blotting, a band of approximately 12 kDa is expected. Consider using appropriate molecular weight markers and optimized lysis buffers for mitochondrial protein extraction.
• In immunofluorescence or immunocytochemistry, mitochondrial localization should be observed. Co-staining with mitochondrial markers is recommended for validation.
• Cross-reactivity with mouse and rat DNAJC19 is documented; however, validation in the relevant sample system is advised when using these species.
• ELISA applications may require titration of the antibody and optimization of the detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated polyclonal antibodies like this DNAJC19 antibody, for mitochondrial biology and protein translocation studies. As part of our comprehensive portfolio, we offer antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development from concept to clinic.