Anti-DNAJC19 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96DA6

Core Product Specifications and Parameters

Biological Background: DNAJC19 Function and Localization

• DNAJC19, also known as mitochondrial import inner membrane translocase subunit TIM14 or DnaJ homolog subfamily C member 19, is a mitochondrial co-chaperone involved in protein translocation and cardiolipin remodeling.
• It functions as a component of the PAM complex, facilitating ATP-dependent translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix.
• DNAJC19 forms a complex with prohibitins to regulate cardiolipin remodeling, essential for mitochondrial membrane integrity.
• Localized to the mitochondrion inner membrane, with a single transmembrane helix.
• Post-translational modifications include acetylation and phosphorylation, indicating regulatory mechanisms.
• Ubiquitously expressed in human tissues, with alternative splicing generating multiple isoforms.
• Mutations in DNAJC19 are associated with dilated cardiomyopathy, highlighting its physiological importance.
• Belongs to the DnaJ family of heat shock proteins, acting as a co-chaperone to stimulate ATP-dependent activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-116) of human DNAJC19. Consider validating antibody specificity using recombinant protein or knockdown/knockout models in your target sample system.
• For Western blot (WB): Run mitochondrial-enriched extracts under reducing conditions; expect a band around 12 kDa. Optimize antibody dilution based on sample type.
• For immunofluorescence/immunocytochemistry (IF/ICC): Permeabilize cells to detect mitochondrial staining. Co-stain with mitochondrial markers (e.g., MitoTracker) to confirm localization.
• For ELISA: Use as a capture or detection antibody after assessing binding affinity to the target antigen or recombinant protein.
• Perform species-reactivity verification if using non-human/mouse/rat samples, as cross-reactivity beyond these species has not been determined.
• Note that the antibody is supplied as a polyclonal rabbit antiserum; lot-to-lot consistency should be monitored for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from development to manufacturing. We provide validated antibodies, including this anti-DNAJC19 polyclonal antibody, optimized for mitochondrial protein import and cardiolipin studies, along with bulk reagents and rare-target sourcing services. Leverage our expertise to accelerate your assay development and supply chain.