Anti-DNAJC18 Rabbit Polyclonal Antibody for WB, ELISA - Q9H819

Core Product Specifications and Parameters

Biological Background: DNAJC18 Function and Localization

• DNAJC18 is a DnaJ/Hsp40 homolog belonging to subfamily C, characterized by a J-domain that mediates interaction with Hsp70 chaperones.
• The primary characterized function involves polyomavirus infection: DNAJC18 is required for virus endoplasmic reticulum membrane penetration by facilitating the recruitment of DNAJB12 and DNAJB14 into SV40-induced foci, which are the sites of viral penetration into the cytosol. Related references: PMID:25631089
• Subcellular localization is at the endoplasmic reticulum membrane, consistent with its role in virus entry.
• The protein is predicted to contain transmembrane helices, anchoring it in the ER membrane.
• UniProt keywords include chaperone, endoplasmic reticulum, membrane, transmembrane, and reference proteome, highlighting its functional and structural classification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 260-350) of DNAJC18, which should generate antibodies recognizing full-length endogenous protein in WB.
• For WB, consider using lysates from human or rat cell lines known to express DNAJC18; validate band specificity using positive controls or knockdown samples.
• For ELISA, optimize coating antigen to match the immunogen sequence for reliable detection.
• Cross-reactivity with other DnaJ family members may occur due to sequence homology; verify in your relevant sample system.
• The predicted molecular weight of DNAJC18 is ~42 kDa; observe a band near this size in reducing SDS-PAGE.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibodies like this anti-DNAJC18 polyclonal antibody for viral infection and chaperone research. Our supply capabilities extend to optimized antibody pairs, bulk ancillary reagents, and sourcing for rare targets, supporting your projects from concept to clinic.