Anti-DNAJC15 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9Y5T4

Core Product Specifications and Parameters

Biological Background: DNAJC15 Function and Localization

• DNAJC15, also known as MCJ (methylation-controlled J protein), is a member of the DnaJ homolog subfamily C and functions as a co-chaperone.
• It acts as a negative regulator of the mitochondrial respiratory chain, preventing hyperpolarization and restricting ATP generation.
• DNAJC15 is a component of the TIM23 translocase complex at the inner mitochondrial membrane, where it stimulates the ATPase activity of mitochondrial HSPA9 (mortalin).
• The protein is localized to the mitochondrial inner membrane and contains a transmembrane helix.
• It belongs to the larger DnaJ co-chaperone family, which typically contains a J domain for interaction with Hsp70 chaperones.
• Tissue expression analysis shows highest levels in heart, followed by liver and kidney.
• Post-translational modifications include phosphorylation, and it is implicated in protein transport and mitochondrial translocation.

Experimental Guidance and Technical Tips

• For Western blotting, consider enriching mitochondrial fractions to improve detection, as DNAJC15 is an inner membrane protein.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions to ensure adequate antibody access to mitochondrial antigens.
• The antibody detects a band at approximately 16 kDa, but post-translational modifications or protein complexes may shift migration.
• The immunogen peptide sequence (aa 70–150) is conserved across human, mouse, and rat, supporting cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this rabbit monoclonal antibody as a validated reagent for mitochondrial research, backed by comprehensive IVD raw material supply capabilities. Whether you need optimized antibody pairs, bulk ancillary reagents, or custom sourcing for rare targets like DNAJC15, our one-stop service supports your project from concept to clinic. Let CamelBio be your trusted partner in diagnostic development.