Anti-DNAJC12 Monoclonal Antibody for WB, ELISA - Q9UKB3

Core Product Specifications and Parameters

Biological Background: DNAJC12 Function and Localization

• DNAJC12 is a member of the DnaJ (Hsp40) co-chaperone family and is also known as J domain-containing protein 1 (JDP1).
• It functions as a probable co-chaperone involved in the proper folding of biopterin-dependent aromatic amino acid hydroxylases, including phenylalanine-4-hydroxylase (PAH), tyrosine 3-monooxygenase (TH), and tryptophan hydroxylases (TPH1 and TPH2).
• DNAJC12 is primarily localized in the cytoplasm.
• The protein is expressed at high levels in the brain, heart, and testis, with lower expression in kidney and stomach.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing produces multiple isoforms.
• Mutations in DNAJC12 are associated with hyperphenylalaninemia and neurological disorders (e.g., HPANBH4).

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was generated against a synthetic peptide spanning amino acids 99–198 of human DNAJC12; it is expected to detect endogenous and recombinant DNAJC12 protein.
• Recommended for western blot (WB) and enzyme-linked immunosorbent assay (ELISA). For WB, a band at approximately 23 kDa is expected; optimize antibody dilution and validate in the relevant sample system.
• The antibody cross-reacts with mouse DNAJC12, as indicated by the supplier; confirm reactivity in your experimental model if needed.
• Consider including appropriate positive and negative controls (e.g., HeLa or HEK293 cell lysates) to verify specificity.

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