Anti-DNAJB9 Polyclonal Antibody for WB and ELISA - Q9UBS3

Core Product Specifications and Parameters

Biological Background: DNAJB9 Function and Localization

• DNAJB9, also known as ERdj4 or MDG1, is a member of the DnaJ homolog subfamily B, encoded by the DNAJB9 gene.
• It functions as a co-chaperone for Hsp70 protein HSPA5/BiP, stimulating its ATPase activity and enabling efficient substrate recognition. Related references: PMID:18400946
• As a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR), DNAJB9 recruits HSPA5/BiP to inactivate ERN1/IRE1 in the unstressed endoplasmic reticulum.
• DNAJB9 is involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins, contributing to protein quality control.
• The protein is localized in the endoplasmic reticulum lumen.
• DNAJB9 is required for survival of B-cell progenitors and normal antibody production.
• Widely expressed, with highest levels in liver, placenta, and kidney. Related references: PMID:11836248
• Keywords: chaperone, endoplasmic reticulum, phosphoprotein, unfolded protein response.

Experimental Guidance and Technical Tips

• The recombinant immunogen corresponds to amino acids 24-223 of human DNAJB9; this region is conserved in mouse and rat, supporting the reported cross-reactivity. Researchers should validate performance in their specific experimental systems.
• For Western blot, a band near 26 kDa is anticipated based on the computed molecular weight. Optimal antibody dilution and incubation conditions should be determined empirically.
• ELISA applications may require optimization of coating antigen and detection conditions; consider testing a range of antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For targets like DNAJB9 involved in ER stress and UPR pathways, we offer tailored sourcing solutions for rare and custom reagents to accelerate assay development from concept to clinic.