Anti-DNAJB6 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - O75190

Core Product Specifications and Parameters

Biological Background: DNAJB6 Function and Localization

• DNAJB6 (DnaJ homolog subfamily B member 6), also known as HSJ-2, MRJ, or MSJ-1, is a member of the Hsp40/DnaJ chaperone family and functions as a co-chaperone of HSP70 by stimulating its ATPase activity in a dose- and time-dependent manner. Related references: PMID:10954706 PMID:28233300

• It plays an indispensable role in the organization of KRT8/KRT18 intermediate filaments, contributing to cytoskeletal integrity. Related references: PMID:10954706

• Acts as an endogenous molecular chaperone for neuronal proteins including huntingtin and suppresses aggregation and toxicity of polyglutamine-containing, aggregation-prone proteins. Related references: PMID:11896048 PMID:22366786 PMID:20159555

• Also reduces cellular toxicity and caspase-3 activity in neurodegenerative contexts. Related references: PMID:11896048

• Isoform B, but not isoform A, specifically inhibits huntingtin aggregation, suggesting isoform‑specific functional differences.

• Subcellular localization includes the cytoplasm (perinuclear region), nucleus, and myofibril sarcomere Z‑line, indicating diverse functional sites.

• Widely expressed with highest levels in testis and brain (hippocampus, thalamus); lower in heart, spleen, intestine, ovary, placenta, lung, kidney, pancreas, thymus, prostate, skeletal muscle, liver, and leukocytes.

• Keywords from UniProt: alternative splicing, chaperone, disease variant (limb‑girdle muscular dystrophy), methylation, phosphoprotein, proteomics identification, and 3D‑structure.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C‑terminal region (aa 177–326) of human DNAJB6, which may recognize both isoforms; consider isoform‑specific controls when investigating isoform‑dependent functions.
• For Western blot, a band around 36 kDa is expected; validate with appropriate positive controls (e.g., HeLa or brain tissue lysates).
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization steps; cross‑reactivity with mouse and rat samples makes this antibody suitable for rodent model studies.
• In ELISA, titrate the antibody to determine optimal working concentration; recombinant protein or peptide blocking may reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a streamlined supply chain for IVD raw materials. For DNAJB6‑related research in chaperone biology, neurodegeneration, and muscular dystrophy, we offer validated antibodies and ancillary reagents to support assay development. Our portfolio includes bulk polyclonal antibodies, monoclonal antibody development, optimized antibody pairs, and specialized sourcing for rare targets, helping accelerate your project from concept to clinic.