Anti-DNAJB1/HSP40 Monoclonal Antibody () for Intracellular Flow Cytometry - P25685

Core Product Specifications and Parameters

Biological Background: DNAJB1/HSP40 Function and Localization

• DNAJB1 (DnaJ homolog subfamily B member 1), also known as Hsp40 or Hdj1, is a member of the DnaJ heat shock protein family and functions as a co-chaperone for HSP70, stimulating its ATPase activity. Related references: PMID:14503850

• It acts as a chaperone adapter together with TTC1, regulating HSP70-dependent protein folding by interacting with the amino terminal region of HSP70. Related references: PMID:14503850

• DNAJB1 stimulates the association between HSC70 and HIP, further modulating chaperone machinery.

• It negatively regulates heat shock-induced transcriptional activity of HSF1 during the attenuation and recovery phase of the heat shock response. Related references: PMID:9499401

• In vitro, DNAJB1 stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B. Related references: PMID:24318877

• The protein localizes to the cytoplasm and nucleus, including the nucleolus, consistent with its role in cellular stress responses. Post-translational modifications include phosphorylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 241–340 of human DNAJB1 (NP_006136.1). This region may be conserved; confirm reactivity in your target species if different from human.
• This antibody is conjugated to and validated for intracellular flow cytometry. Ensure appropriate fixation and permeabilization protocols are employed for detection of intracellular DNAJB1.
• Cross-reactivity is reported for human; verify performance in other species or sample types as needed.

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CamelBio supports diagnostic manufacturers and research laboratories with comprehensive sourcing of IVD raw materials. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your assay development. For researchers investigating chaperone biology and stress response pathways, we can assist in sourcing rare-target raw materials and technical consultation to accelerate your project from concept to clinic.