Anti-DNAJB14 Rabbit Polyclonal Antibody for WB and ELISA - Q8TBM8

Core Product Specifications and Parameters

Biological Background: DNAJB14 Function and Localization

• DNAJB14 is an Hsp40/DnaJ co-chaperone that partners with HSPA8/Hsc70 to control ATP hydrolysis and substrate binding, essential for protein folding and transport. Related references: PMID:24732912
• It directs misfolded proteins to the endoplasmic reticulum-associated degradation (ERAD) pathway, preventing toxic protein aggregation. Related references: PMID:24732912
• Together with DNAJB12, DNAJB14 facilitates the maturation of voltage-gated potassium channels KCND2 and KCNH2 by stabilizing nascent subunits and promoting their tetrameric assembly, a process partially independent of HSPA8/Hsc70. Related references: PMID:27916661
• Upon overexpression, DNAJB12 and DNAJB14 form nuclear membranous structures with HSPA8/Hsc70, termed DJANGOs, of yet unknown function. Related references: PMID:24732912
• During polyomavirus infection, DNAJB14 is hijacked to facilitate virus penetration of the endoplasmic reticulum membrane and subsequent infection. Related references: PMID:21673190, PMID:24675744
• The protein localizes to the endoplasmic reticulum membrane and the nuclear membrane, reflecting its roles in protein quality control and nuclear structure formation.
• DNAJB14 exhibits key structural features including a J-domain for Hsc70 interaction, transmembrane helices for membrane anchoring, and alternatively spliced variants.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–120) targets the N-terminal region of DNAJB14, which includes part of the J-domain; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, consider using whole-cell lysates from cell lines of human, mouse, or rat origin; nuclear enrichment protocols may enhance detection due to DNAJB14's nuclear membrane localization.
• For ELISA, validate the optimal coating concentration and detection antibody pair empirically; this antibody is suitable as a capture or detection reagent.
• As a polyclonal antibody, lot-to-lot consistency may vary; confirm performance with appropriate positive and negative controls in the relevant sample system.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated IVD raw materials, including this anti-DNAJB14 polyclonal antibody for protein quality control and viral infection studies. Our comprehensive portfolio spans optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your raw material procurement from discovery to commercialization.