Anti-DNAJB12 Rabbit Polyclonal Antibody for WB & ELISA - Q9NXW2

Core Product Specifications and Parameters

Biological Background: DNAJB12 Function and Localization

• DNAJB12 (DnaJ homolog subfamily B member 12), also known as DJ10, is a member of the DnaJ/Hsp40 family of co-chaperones.
• Acts as a co-chaperone with HSPA8/Hsc70, promoting protein folding and trafficking, preventing aggregation, and targeting unfolded proteins to the ERAD pathway. Related references: PMID:21148293, PMID:21150129
• Together with DNAJB14, functions as a chaperone for the maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent subunits and facilitating their oligomerization, independently of HSPA8/Hsc70. Related references: PMID:27916661
• Overexpression of DNAJB12 leads to formation of nuclear membranous structures (DJANGOs) with DNAJB14 and HSPA8/Hsc70, whose cellular role remains unclear. Related references: PMID:24732912
• During polyomavirus infection, DNAJB12 is involved in virus endoplasmic reticulum membrane penetration and infection. Related references: PMID:21673190, PMID:24675744
• Localizes to the endoplasmic reticulum membrane and nucleus membrane.
• Post-translational modifications include acetylation and methylation; the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen was raised against amino acids 250-409 of human DNAJB12; consider using the antibody to detect endogenous DNAJB12 in human, mouse, or rat lysates by Western blot.
• For ELISA, validate optimal antibody dilution and antigen coating conditions using recombinant DNAJB12 protein or appropriate cell lysates.
• DNAJB12 has a predicted molecular weight of ~42 kDa; however, post-translational modifications may cause slight band shifts—validate with positive controls.
• Given cross-reactivity with mouse and rat, ensure species-specific validation if required for non-human studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive one-stop platform for IVD raw materials. This anti-DNAJB12 antibody supports studies on protein folding, ER-associated degradation, and ion channel maturation. Beyond this product, we offer validated antibody pairs, bulk reagents, and rare-target sourcing—bridging the gap from concept to clinical application efficiently.