Anti-DNAJB11 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UBS4

Core Product Specifications and Parameters

Biological Background: DNAJB11 Function and Localization

• DNAJB11, also known as ERdj3, ABBP-2, and hDj-9, is a member of the DnaJ (Hsp40) co-chaperone family localized to the endoplasmic reticulum lumen.
• As a co-chaperone for HSPA5, DNAJB11 is essential for proper protein folding, trafficking, and ER-associated degradation (ERAD) of substrates. Related references: PMID:10827079, PMID:15525676, PMID:29706351
• It recognizes and binds directly to unfolded proteins and nascent polypeptide chains, recruiting HSPA5 and other chaperones to the substrate. Related references: PMID:15525676
• DNAJB11 stimulates the ATPase activity of HSPA5, driving the chaperone cycle. Related references: PMID:10827079
• The protein is necessary for the maturation and correct trafficking of polycystin-1 (PKD1), linking it to polycystic kidney disease. Related references: PMID:29706351
• It is widely expressed across human tissues, consistent with its fundamental role in ER protein quality control.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, as indicated by proteomics and sequence analysis.
• Alternative names such as PKD6, ABBP-2, and ERdj3 reflect its involvement in diverse cellular processes.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 89–358 of human DNAJB11, targeting a central region that may be conserved across species; thus, cross-reactivity with mouse and rat is supported by the supplier.
• For western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines, and optimize antibody dilution empirically.
• In immunohistochemistry (IHC-P), antigen retrieval steps should be validated in the target tissue type, and a suitable detection system should be employed.
• ELISA can be used for quantitative detection of DNAJB11 in biological fluids; pairing with a matched detection antibody is recommended for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides DNAJB11 polyclonal antibodies and complementary reagents for ER proteostasis and polycystic kidney disease research. As a one-stop IVD raw material supplier, we offer validated antibody pairs, bulk ancillary reagents, and access to rare target raw materials to streamline your diagnostic development from concept to clinic.