Anti-DNAJA2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60884

Core Product Specifications and Parameters

Biological Background: DNAJA2 Function and Localization

• DNAJA2 is a member of the DnaJ (Hsp40) family of co-chaperones, sharing homology with the bacterial DnaJ protein.
• It functions as a co-chaperone of Hsc70 (HSPA8), stimulating ATP hydrolysis and facilitating the folding of unfolded proteins, as shown in vitro with HSPA1A/B. Related references: PMID:24318877
• The protein is also known by several aliases, including DJ3, CPR3, HIRIP4 (HIRA-interacting protein 4), and renal carcinoma antigen NY-REN-14, reflecting its identification in various cellular and cancer contexts.
• DNAJA2 is localized to membrane structures, indicating a role in membrane-associated protein quality control or trafficking.
• Post-translational modifications include acetylation, phosphorylation, methylation, and prenylation, which may regulate its activity and cellular distribution.
• The protein contains a zinc-finger domain, suggesting involvement in zinc-dependent processes and potential interactions with nucleic acids or other proteins.
• As a molecular chaperone, DNAJA2 participates in preventing protein aggregation and maintaining proteostasis under stress conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 233-412 of human DNAJA2, a region that may be conserved across species, supporting cross-reactivity with mouse and rat as indicated.
• For Western blot, the predicted molecular weight is approximately 46 kDa (UniProt: O60884); consider running appropriate positive controls (e.g., HeLa, HEK293 cells) and using a reducing gel.
• In IHC-P and IF/ICC, validate the antibody in your specific tissue or cell type, and optimize antigen retrieval and blocking steps, as membrane localization may require permeabilization.
• For ELISA, the antibody can be explored as a capture or detection reagent; pair with a suitable monoclonal antibody for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined pathway to high-quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For targets like DNAJA2, a key co-chaperone in protein folding and membrane biology, we provide optimized polyclonal antibodies alongside custom sourcing solutions to meet your assay development needs. Partner with CamelBio for efficient, end-to-end support from concept to clinic.