Anti-DNA topoisomerase II alpha (TOP2A) Rabbit Monoclonal Antibody for WB - P11388

Core Product Specifications and Parameters

Biological Background: DNA topoisomerase II alpha (TOP2A) Function and Localization

• TOP2A encodes DNA topoisomerase II alpha, an ATP-dependent decatenating enzyme that resolves topological stress during DNA replication and transcription by transiently breaking and rejoining double-stranded DNA. Related references: PMID:17567603 PMID:18790802 PMID:22013166
• The protein localizes primarily to the nucleoplasm and nucleolus during interphase and associates with chromosomes during mitosis, with some cytoplasmic distribution.
• TOP2A is a phosphoprotein subject to multiple post-translational modifications, including acetylation, isopeptide bond formation, and ubiquitination, which regulate its catalytic activity and stability.
• Tissues with high proliferative index, such as tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain, and lung, express TOP2A, and it is particularly elevated in high-grade lymphomas, squamous cell lung tumors, and seminomas. Related references: PMID:9155056
• As a member of the type II topoisomerase family, TOP2A requires ATP and magnesium for its catalytic cycle and is a target for several anticancer drugs.
• Alternative splicing generates multiple isoforms with distinct molecular weights.
• TOP2A may also participate in circadian rhythm regulation by modulating the period length of BMAL1 transcriptional oscillation.

Experimental Guidance and Technical Tips

• The immunogen spans a conserved C-terminal region, suggesting that the antibody may detect multiple splice variants or isoforms. Researchers should confirm specificity via peptide blocking or siRNA knockdown.
• For IHC applications, antigen retrieval methods and tissue fixation conditions can influence staining; optimization using positive (e.g., tonsil, high-grade lymphoma) and negative controls is recommended.
• In IF experiments, nuclear localization is expected; counterstaining with DAPI can help confirm target specificity.
• This antibody is supplied as a monoclonal rabbit IgG; use appropriate secondary antibodies for detection.

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