Anti-DNA topoisomerase II alpha (TOP2A) Polyclonal Antibody for IF/ICC, ELISA - P11388

Core Product Specifications and Parameters

Biological Background: DNA topoisomerase II alpha (TOP2A) Function and Localization

• DNA topoisomerase II alpha is a key decatenating enzyme that alters DNA topology by generating transient double-strand breaks, passing an intact DNA duplex through the break, and religating the broken strand. Related references: PMID:17567603, PMID:18790802, PMID:22013166
• It may play a role in regulating the period length of BMAL1 transcriptional oscillation.
• TOP2A is expressed in a variety of tissues including tonsil, spleen, lymph node, thymus, skin, pancreas, testis, colon, kidney, liver, brain, and lung, as well as in high-grade lymphomas, squamous cell lung tumors, and seminomas. Related references: PMID:9155056
• The protein localizes to the cytoplasm, nucleoplasm, and nucleolus.
• TOP2A belongs to the type II topoisomerase family; it requires ATP binding and magnesium as a cofactor for its catalytic activity.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• Multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal domain (aa 1314-1503), which is unique to TOP2A and not present in the beta isoform; this may confer isoform specificity.
• For immunofluorescence/immunocytochemistry (IF/ICC), consider fixation and permeabilization conditions that preserve nuclear and nucleolar architecture.
• For ELISA, the antibody may be used for detection of recombinant or native TOP2A; validate dilution and assay conditions in the specific experimental system.
• Since TOP2A is expressed in proliferating cells and certain tumors, appropriate positive and negative controls should be included in experiments.

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