Anti-DNA topoisomerase I (TOP1) Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - P11387

Core Product Specifications and Parameters

Biological Background: DNA topoisomerase I Function and Localization

• DNA topoisomerase I (TOP1) is an enzyme encoded by the TOP1 gene, with a molecular weight of approximately 91 kDa (UniProt: P11387). It is also known as DNA topoisomerase I.
• TOP1 relieves DNA supercoiling and torsional tension during replication and transcription by transiently cleaving one strand of DNA, forming a covalent intermediate, rotating the free strand, and then religating the break.
• It regulates alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells, highlighting a role in coagulation-related gene expression.
• TOP1 contributes to circadian rhythm regulation by modulating chromatin structure at ROR response elements on the BMAL1 promoter, influencing core clock gene transcription.
• The protein localizes to the nucleus, with presence in both the nucleolus and nucleoplasm.
• TOP1 is expressed in endothelial cells, among other tissues.
• Post-translational modifications include acetylation and phosphorylation, and it has been implicated as a proto-oncogene in chromosomal rearrangements.
• It is a phosphoprotein and is subject to ubiquitination and isopeptide bond formation, reflecting complex regulatory control.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), so the antibody likely recognizes the full-length protein. For Western blot, expect a band near 91 kDa; consider using appropriate loading controls and optimizing blocking conditions.
• For IHC-P, antigen retrieval methods may be necessary to expose the epitope; validate staining in known positive tissues such as those high in endothelial cells.
• In immunoprecipitation, verify capture efficiency in relevant cell lysates expressing TOP1, and use appropriate negative controls (e.g., IgG).
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a matched antibody if needed and validate sensitivity with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop platform for IVD raw materials, technical services, and consulting. This DNA topoisomerase I (TOP1) antibody supports research into DNA topology, chromatin dynamics, and transcriptional regulation. Explore our portfolio for validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare targets to accelerate your assay development from concept to clinical application.