Anti-DNA Polymerase beta Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06746

Core Product Specifications and Parameters

Biological Background: DNA Polymerase beta Function and Localization

• DNA polymerase beta (POLB), also known as 5'-deoxyribose-phosphate lyase (5'-dRP lyase) or AP lyase, is a repair polymerase that plays a key role in base-excision repair (BER). This pathway corrects damaged bases, and POLB is essential for the repair synthesis step. Related references: PMID:10556592, PMID:9207062, PMID:9572863
• Following base excision and strand nicking by an AP endonuclease, POLB removes the 5'-deoxyribose-phosphate group from the pre-incised AP site via its 5'-dRP lyase activity. It then fills the single-nucleotide gap through its DNA polymerase activity. Related references: PMID:10556592, PMID:17526740, PMID:8841119
• POLB also exhibits AP lyase activity, cleaving sugar-phosphate bonds 3' to an intact abasic (AP) site, thereby contributing to alternative repair pathways. Related references: PMID:9614142
• The polymerase activity of POLB is distributive rather than processive, adding one nucleotide at a time in a stepwise manner, which is distinct from replicative DNA polymerases.
• POLB localizes to both the nucleus and cytoplasm, consistent with its roles in nuclear DNA repair and possible cytoplasmic functions.
• Post-translational modifications of POLB include acetylation, methylation, and ubiquitin conjugation, which may regulate its stability, activity, or interaction with other BER proteins.
• POLB is a key component of the cellular response to DNA damage, contributing to genome stability and playing roles in processes such as DNA replication and repair.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human POLB protein (amino acids 1–335), which may elicit antibodies against multiple epitopes. Consider this broad reactivity when interpreting results.
• For Western blot, a specific band around 38 kDa is expected. Validate with appropriate positive and negative controls, and consider using KO-validated lysates to confirm specificity.
• This antibody is suitable for IHC-P, IF/ICC, and ELISA. Optimize dilutions and antigen retrieval conditions experimentally for each application and sample type.
• Cross-reactivity with mouse POLB is reported, enabling studies in mouse models. However, verify cross-reactivity in your specific experimental system.
• As a polyclonal antibody, lot-to-lot consistency should be monitored. Include internal reference standards for quantitative comparisons across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-POLB reagent, and bulk ancillary reagents to support your assay development. Whether you are studying DNA repair pathways, developing cancer diagnostics, or require rare target raw materials, CamelBio streamlines procurement from concept to clinic. Our global network ensures reliable supply and technical consultation for diagnostic manufacturers and research laboratories.