Anti-DNA Polymerase beta Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P06746

Core Product Specifications and Parameters

Biological Background: DNA Polymerase beta Function and Localization

• DNA Polymerase beta (POLB) is a repair polymerase essential for base-excision repair (BER). It possesses 5'-deoxyribose-phosphate lyase (dRP lyase) activity to remove the 5'-dRP group from preincised AP sites, and DNA polymerase activity to fill single-nucleotide gaps with the correct nucleotide. Related references: PMID:10556592 PMID:9207062 PMID:9572863
• POLB conducts gap-filling DNA synthesis in a stepwise distributive manner, adding one nucleotide per binding event, unlike processive polymerases. Related references: PMID:17526740 PMID:8841119
• Beyond its polymerase and dRP lyase activities, POLB can also act as an AP lyase, cleaving sugar-phosphate bonds 3' to an intact abasic site. Related references: PMID:9614142
• Subcellular localization includes both the nucleus and cytoplasm, consistent with its role in nuclear DNA repair and potential cytoplasmic DNA damage responses.
• Post-translational modifications include acetylation, methylation, and ubiquitin-like conjugation, which may regulate its stability and function.
• As a key player in the DNA damage response, POLB is implicated in maintaining genomic stability, and its deficiency is associated with increased sensitivity to alkylating agents.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 236–335 of human POLB; antibody performance should be validated in the specific sample system and application of interest.
• For Western blotting, a band at approximately 38 kDa is expected; consider using appropriate positive and negative controls.
• IHC-P experiments may require optimized antigen retrieval protocols; preliminary testing on positive control tissues (e.g., human tonsil) is recommended.
• The antibody is suitable for ELISA; it is advisable to determine optimal working dilutions by titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-DNA Polymerase beta rabbit monoclonal antibody as part of our extensive IVD raw material portfolio. In addition to validated antibody pairs and optimized monoclonal/polyclonal antibodies, we offer bulk ancillary reagents and can assist with sourcing rare-target raw materials for DNA repair and genomic stability research. Let us be your one-stop bridge from concept to clinic.