Anti-DNA-PKcs Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P78527

Core Product Specifications and Parameters

Biological Background: DNA-PKcs (DNA-dependent protein kinase catalytic subunit) Function and Localization

• DNA-PKcs is the catalytic subunit of the DNA-dependent protein kinase (DNA-PK) complex, encoded by the PRKDC gene (also known as HYRC, HYRC1, DNPK1, p460).
• As a molecular sensor for DNA damage, DNA-PKcs is essential for non-homologous end joining (NHEJ), the primary pathway repairing double-strand breaks (DSBs) and V(D)J recombination. Related references: PMID:11955432, PMID:12649176, PMID:14734805
• DNA-PKcs requires DNA binding for catalytic activation; it is recruited to DNA ends by the Ku70/Ku80 heterodimer (XRCC6/XRCC5) to protect and align broken DNA ends and sequester the break for NHEJ. Related references: PMID:11955432, PMID:12649176, PMID:14734805
• Beyond DNA repair, DNA-PKcs acts as a scaffold protein, modulating transcription, promoting ribosome assembly by processing precursor rRNA, and maintaining telomeric stability. Related references: PMID:11955432, PMID:12649176, PMID:14734805, PMID:32103174
• DNA-PKcs is primarily localized in the nucleus and nucleolus, with additional presence in the cytosol.
• As a serine/threonine kinase recognizing the [ST]-Q consensus, DNA-PKcs phosphorylates key substrates including H2AX (Ser-139) for DNA damage signaling, AKT (Ser-473) for PI3K/AKT pathway regulation, and numerous DNA repair factors (e.g., XRCC4, XRCC5, XRCC6). Related references: PMID:11955432, PMID:12649176, PMID:14734805, PMID:14627815, PMID:16046194, PMID:15262962

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 3987-4086 of human DNA-PKcs; expect reactivity near the C-terminal region of the protein.
• For Western blot (WB), the predicted molecular weight is approximately 469 kDa; consider using low-percentage SDS-PAGE gradient gels and extended transfer times to ensure efficient blotting of this large protein.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validation of antigen retrieval methods and fixation conditions in relevant tissue or cell samples is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed by the supplier; always include appropriate positive and negative controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic developers and research laboratories with a seamless supply chain for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Our anti-DNA-PKcs polyclonal antibody supports investigations into DNA damage repair and kinase signaling pathways. Beyond this product, CamelBio offers tailored sourcing solutions for rare targets and integrated technical support to streamline your assay development—from concept to clinic.