Anti-DNA-PKcs Polyclonal Antibody for WB, IHC-P, ELISA - P78527

Core Product Specifications and Parameters

Biological Background: DNA-PKcs Function and Localization

• Serine/threonine-protein kinase acting as a molecular sensor for DNA damage, essential for DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Catalytic activity requires DNA binding. Related references: PMID:11955432, PMID:12649176, PMID:14734805
• Recruited by XRCC5/XRCC6 to DNA ends, protecting and aligning broken ends, preventing degradation, and sequestering the DSB for repair by NHEJ. Acts as a scaffold for DNA repair protein localization. Related references: PMID:11955432, PMID:12649176, PMID:14734805
• Phosphorylates key substrates in DNA damage response, including H2AX ('Ser-139'), p53/TP53, c-Abl/ABL1, and artemis (DCLRE1C), modulating their functions. Related references: PMID:14627815, PMID:16046194, PMID:10026262, PMID:10467406, PMID:11889123
• Involved in modulation of transcription and ribosome biogenesis: promotes processing of precursor rRNA into mature 18S rRNA within the small-subunit processome via binding to U3 snoRNA. Related references: PMID:32103174
• Contributes to circadian rhythm regulation by antagonizing CRY1 phosphorylation and increasing CRY1 protein stability, likely through an indirect mechanism. (By similarity)
• Regulates innate immune response through the cGAS-STING pathway by assembling into the HDP-RNP complex and phosphorylating CGAS, impairing its oligomerization. Related references: PMID:28712728, PMID:33273464
• Localizes primarily to the nucleus, nucleolus, and cytosol. Found at chromosome ends, suggesting a role in telomere maintenance.
• Keywords: DNA damage, DNA repair, DNA-binding, Kinase, Serine/threonine-protein kinase, Nucleus, Innate immunity, Disease variant (IMD26).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (a.a. 3993-4128) of human DNA-PKcs; verify recognition of the full-length protein in relevant sample systems.
• For Western blot, due to the large molecular weight (469 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6% or gradient) and extended transfer times. Confirm specificity with appropriate controls.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0 or Tris-EDTA pH 9.0) and test multiple concentrations to reduce background.
• In ELISA applications, validate the antibody’s reactivity with the immunogen or native protein under various coating conditions.
• Cross-reactivity is reported for human; test reactivity with other species if needed, as sequence homology may exist.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes one-stop access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-DNA-PKcs polyclonal antibody. Whether you need bulk ancillary reagents, rare target sourcing, or support for DNA repair and oncology research, our supply chain covers every stage from concept to clinic. Partner with us to streamline your procurement for robust and reproducible assay development.