Anti-DMP1 Polyclonal Antibody for WB and ELISA - Q13316

Core Product Specifications and Parameters

Biological Background: DMP1 Function and Localization

• DMP1 (Dentin Matrix Acidic Phosphoprotein 1), also designated ARHP, ARHR, and DMP-1, is a member of the SIBLING (Small Integrin-Binding Ligand, N-linked Glycoprotein) family of extracellular matrix proteins.
• This protein has a dual function in osteoblast differentiation: in its unphosphorylated form, it localizes to the nucleus of undifferentiated osteoblasts and acts as a transcriptional regulator for osteoblast-specific genes such as osteocalcin.
• Upon phosphorylation during the osteoblast-to-osteocyte transition, DMP1 is exported to the extracellular matrix, where it regulates the nucleation of hydroxyapatite crystals, playing a central role in biomineralization.
• DMP1 is highly expressed in mineralized tissues, with prominent expression in tooth (odontoblasts, ameloblasts, and cementoblasts) and bone.
• The protein is subject to alternative splicing, phosphorylation, and glycosylation, consistent with its multifaceted roles in the nucleus, cytoplasm, and extracellular matrix.
• Its predicted molecular weight is approximately 56 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human DMP1. Because this region is conserved across human, mouse, and rat, the antibody is expected to cross-react; validate reactivity in your specific model system.
• For Western blot, a primary band near 56 kDa is anticipated. Due to phosphorylation and other post-translational modifications, additional bands may appear; consider including phosphatase inhibitors in lysis buffers.
• For ELISA, the antibody can be applied in both direct and sandwich formats. Titration of the antibody and optimization of blocking conditions are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, supporting diagnostic manufacturers, research laboratories, and clinical developers from early-stage concept through to clinical application. This anti-DMP1 polyclonal antibody is part of our extensive portfolio for bone and tooth mineralization research, backed by our ability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need consistent performance in established assays or assistance with niche marker sourcing, CamelBio bridges the gap with reliable, scalable supply.