Anti-DMD Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P11532

Core Product Specifications and Parameters

Biological Background: Dystrophin Function and Localization

• Dystrophin (DMD gene) is a large 427 kDa cytoskeletal protein that anchors the extracellular matrix to the actin cytoskeleton via its interaction with dystroglycan.
• It is a core component of the dystrophin-associated glycoprotein complex (DAPC) that accumulates at the neuromuscular junction and various synapses, providing structural stability to the sarcolemma.
• Dystrophin is also implicated in cell signaling and synaptic transmission events.
• Subcellularly, it localizes to the cell membrane (sarcolemma), cytoplasm, cytoskeleton, and postsynaptic cell membrane.
• It is predominantly expressed in muscle fibers at costameres of the sarcolemma, as well as in brain, kidney, lung, and testis; isoform 15 is uniquely detected in heart and liver.
• The protein contains multiple domains including actin-binding and zinc-finger motifs, is subject to phosphorylation, and binds calcium and zinc, reflecting its regulatory roles.
• Mutations in DMD cause Duchenne/Becker muscular dystrophy and cardiomyopathy, underscoring its critical role in muscle homeostasis.

Experimental Guidance and Technical Tips

• For Western blotting, due to the large molecular weight (427 kDa), consider using a low-percentage polyacrylamide gel (e.g., 6%) and optimize electroblotting conditions (e.g., extended transfer time) to ensure efficient transfer of dystrophin.
• In immunofluorescence, anticipate sarcolemmal/peripheral membrane staining in muscle tissues and costamere-enriched patterns; use appropriate positive controls such as skeletal muscle samples.
• The antibody is reactive against human, mouse, and rat; validate performance in your specific sample system to confirm species cross-reactivity under your experimental conditions.
• As a polyclonal reagent, each lot may exhibit slight variations; we recommend titration and inclusion of relevant controls for consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. For neuromuscular disease research and dystrophin-related investigations, we provide validated primary and secondary antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Our robust supply chain ensures consistent quality and helps accelerate your assay development and validation workflows.