Anti-DMC1 Polyclonal Antibody for WB and ELISA - Q14565

Core Product Specifications and Parameters

Biological Background: DMC1 Function and Localization

• DMC1 is a member of the RecA/Rad51 family of recombinases and is essential for meiotic homologous recombination.
• DMC1 catalyzes ATP-dependent DNA strand exchange during meiosis, promoting the pairing and exchange of homologous chromosomes. Related references: PMID:15164066 PMID:21307306 PMID:26976601
• Unlike RAD51, DMC1 tolerates mismatches in heteroduplex DNA and preferentially uses the homologous chromosome rather than the sister chromatid as a repair template. Related references: PMID:15164066 PMID:26976601
• DMC1 localizes to chromosomes and the nucleus, consistent with its role in meiotic DNA repair.
• The DMC1 gene undergoes alternative splicing, yielding multiple transcript variants.
• DMC1 contains an ATP-binding domain and exhibits DNA-dependent ATPase activity, as highlighted by its functional keywords.
• The full-length human DMC1 protein is approximately 38 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-260 of human DMC1 (NP_008999.2), suggesting the antibody recognizes the N-terminal region. It may detect both full-length and N-terminal fragments.
• For Western blotting, expect a predominant band around 38 kDa, corresponding to the predicted molecular weight of DMC1. Validate in your specific sample and lysis conditions.
• Cross-reactivity with human, mouse, and rat indicates potential utility across species, but performance should be verified individually.
• Consider appropriate blocking and dilution conditions for ELISA, as these can vary with antigen coating and detection methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD manufacturers and research laboratories, providing one-stop access to high-quality raw materials including antibodies, antigen pairs, and ancillary reagents. This anti-DMC1 polyclonal antibody is part of our portfolio supporting meiosis and DNA repair research, and we can assist with bulk orders, alternative clone options, or custom raw material sourcing to meet your assay development needs. From concept to clinical application, CamelBio bridges critical supply gaps with efficient, reliable solutions.