Anti-DMAP1 Polyclonal Antibody for WB, ELISA - Q9NPF5

Core Product Specifications and Parameters

Biological Background: DMAP1 Function and Localization

• DNA methyltransferase 1-associated protein 1 (DMAP1) is a dual-function transcription regulator that acts as both a repressor and activator of gene expression.
• DMAP1 interacts with HDAC2, providing a mechanism for histone deacetylation in heterochromatin following DNA replication at late-firing origins, and can also repress transcription independently of histone deacetylase activity.
• As a core component of the NuA4 histone acetyltransferase (HAT) complex, DMAP1 contributes to transcriptional activation by acetylating nucleosomal histones H4 and H2A, thereby modulating chromatin structure and promoting positive transcriptional regulation.
• The NuA4 complex is implicated in diverse cellular processes including oncogene- and proto-oncogene-mediated growth induction, tumor suppressor-mediated growth arrest, replicative senescence, apoptosis, and DNA repair.
• DMAP1 participates in the nuclear localization of URI1 and enhances its transcriptional corepressor activity.
• Subcellular fractionation studies localize DMAP1 to both the nucleus and the cytoplasm.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitination (isopeptide bond), and contains a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–305) of human DMAP1, which may recognize full-length DMAP1 and potentially cross-react with conserved regions in mouse and rat.
• For Western blotting, a band at approximately 53 kDa is expected; consider optimizing blocking and antibody dilution using lysates from human, mouse, or rat cell lines expressing DMAP1.
• ELISA-based detection may require titration of the antibody to establish optimal signal-to-noise ratios; validate performance in the relevant sample matrix.
• If nonspecific bands appear, consider using recombinant DMAP1 or a positive control lysate to verify specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, offering a streamlined supply chain for antibodies, antigens, and ancillary reagents. This anti-DMAP1 polyclonal antibody supports chromatin biology and transcription research applications. Our team can assist in sourcing validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target reagents to accelerate your diagnostic development.