Anti-DLX5 Polyclonal Antibody for WB, ELISA - P56178

Core Product Specifications and Parameters

Biological Background: DLX5 Function and Localization

• DLX5 is a homeobox-containing transcription factor that plays a crucial role in bone development by acting as an immediate early BMP-responsive activator essential for osteoblast differentiation.
• It stimulates the promoter activity of ALPL (alkaline phosphatase) and SP7 (osterix) in a RUNX2-independent manner, driving the transcriptional program of bone-forming cells.
• DLX5 binds to specific homeodomain-response elements in target gene promoters, with the consensus DNA-binding sequence 5'-TAATTA-3'.
• It localizes to the nucleus, consistent with its function as a transcriptional regulator.
• Keywords from UniProt include: Alternative splicing, Deafness, Developmental protein, Disease variant, DNA-binding, Homeobox, Nucleus, Osteogenesis, Phosphoprotein, and Transcription regulation, highlighting its multifunctional role in development and disease.
• DLX5 is also implicated in cell proliferation through up-regulation of MYC promoter activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (amino acids 200 to end) of human DLX5, so the antibody may recognize full-length protein and potentially some isoforms.
• For Western blot, consider using osteoblast-like cell lines (e.g., Saos-2, MG-63) or bone tissue lysates where DLX5 expression is expected; endogenous levels may be low in non-osseous tissues.
• Cross-reactivity with mouse and rat has been reported; validate species-specific performance in your experimental system using appropriate controls.
• In ELISA applications, optimize coating conditions and antibody dilutions based on the peptide immunogen to ensure sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement channel for IVD manufacturers and research laboratories seeking high-quality raw materials. This anti-DLX5 polyclonal antibody supports bone development and transcription factor research, available alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. We help you bridge concept-to-clinic with reliable, science-backed sourcing solutions.