Anti-DLST Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P36957

Core Product Specifications and Parameters

Biological Background: DLST Function and Localization

• DLST (dihydrolipoamide S-succinyltransferase) is the E2 component of the 2-oxoglutarate dehydrogenase complex, also known as OGDC-E2 or E2K. It catalyzes the transfer of a succinyl group from dihydrolipoamide to coenzyme A, forming succinyl-CoA.
• The 2-oxoglutarate dehydrogenase complex converts 2-oxoglutarate to succinyl-CoA and CO2, a critical step in the tricarboxylic acid (TCA) cycle. Related references: PMID:29211711, PMID:30929736
• A fraction of DLST localizes to the nucleus, where it associates with chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A, facilitating histone succinylation. Related references: PMID:29211711
• The protein is predominantly mitochondrial (matrix) but is also found in the nucleus. It contains lipoyl domains essential for its enzymatic function.
• DLST undergoes post-translational modifications including acetylation and phosphorylation, which may modulate its activity and interactions.
• Alternative splicing produces multiple isoforms, potentially allowing tissue-specific regulation or distinct functional roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 150–250 of human DLST. For Western blot, expect a band near 49kDa; validate in human cell lines known to express DLST, such as HeLa or HEK293.
• In IF/ICC and IHC-P, anticipate both mitochondrial and nuclear staining patterns due to the dual localization of DLST. Use appropriate organelle markers for confirmation.
• For ELISA, consider coating with recombinant DLST protein or validated peptide; cross-reactivity is limited to human, so test assay specificity against other species if needed.
• Since this is a monoclonal antibody, lot-to-lot consistency should be high; nevertheless, perform routine quality checks in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-DLST monoclonal antibody supports studies on TCA cycle metabolism and histone succinylation, and we can assist with sourcing rare-target raw materials and custom reagent development. Let CamelBio be your reliable partner from concept to clinic, streamlining your procurement and accelerating your assay development.