Anti-DLST Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36957

Core Product Specifications and Parameters

Biological Background: DLST Function and Localization

• DLST is the dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex (OGDC), also known as E2K.
• The OGDC catalyzes the conversion of 2-oxoglutarate to succinyl-CoA and CO2, a key reaction in the tricarboxylic acid (TCA) cycle. Related references: PMID:29211711 PMID:30929736
• The OGDC is primarily localized to the mitochondrial matrix, where it functions in energy metabolism.
• A fraction of DLST localizes to the nucleus, where it interacts with KAT2A and provides succinyl-CoA for histone succinylation, a chromatin modification. Related references: PMID:29211711
• DLST contains lipoyl domains essential for its catalytic activity and is subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing of DLST yields multiple isoforms, potentially influencing complex assembly or localization.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 150–250 of human DLST (NP_001924.2); epitope mapping suggests this region may be exposed in native and denatured conformations.
• For western blot (WB), DLST migrates at approximately 49 kDa; denaturing and reducing conditions are recommended. Validate expression in mitochondrial-rich tissues (e.g., liver, kidney) or cell lines positive for OGDC components.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval may be required for formalin-fixed paraffin-embedded sections. Subcellular staining (mitochondrial and nuclear) should be assessed against known localization patterns.
• The antibody shows cross-reactivity with mouse and rat; verify protocol compatibility when switching species.
• For ELISA applications, consider using the immunogen peptide as a positive control to confirm activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-DLST antibody serves mitochondrial biology and epigenetic research needs, supporting applications from biomarker discovery to diagnostic assay development. Our supply capabilities extend to rare-target raw-material sourcing, ensuring a streamlined path from concept to clinic.