Anti-DLL4 Rabbit Polyclonal Antibody for WB, ELISA - Q9NR61

Core Product Specifications and Parameters

Biological Background: DLL4 Function and Localization

• DLL4 (Delta-like protein 4, also known as Delta4) is a transmembrane ligand of the Notch signaling pathway, activating NOTCH1 and NOTCH4 receptors to mediate cell fate decisions. Related references: PMID:11134954

• It plays a critical role in angiogenesis by negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. Related references: PMID:20616313

• During spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate, contributing to neuronal subtype specification. Related references: PMID:17728344

• In retinal development, DLL4 is essential for progenitor cell proliferation and suppresses rod photoreceptor fates to ensure proper generation of other retinal cell types (By similarity).

• Subcellularly, DLL4 localizes to the cell membrane and contains extracellular EGF-like domains, a transmembrane helix, and a cytoplasmic tail.

• DLL4 is specifically expressed in vascular endothelium, consistent with its primary role in vascular biology and angiogenesis.

• Post-translational modifications include disulfide bonds and glycosylation, and the protein is associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 27–524), suggesting the antibody may detect the full-length DLL4 protein. Validate performance in your specific sample system.
• For Western blotting, consider testing both reduced and non-reduced conditions due to the presence of disulfide bonds. A band around 74 kDa is expected.
• In ELISA, this antibody may be suitable as a capture or detection reagent for DLL4. Optimize coating conditions and validate with appropriate standards.
• Endothelial cell lysates (e.g., HUVECs) or tissues with active angiogenesis (e.g., placenta, tumor) can serve as positive controls given DLL4's expression pattern.
• Cross-reactivity is limited to human; verify species reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials. For Notch signaling and angiogenesis studies, we supply validated DLL4 antibodies, recombinant proteins, and ancillary reagents. From bulk quantities to rare target sourcing, CamelBio is your trusted partner from concept to clinic.