Anti-DLL4 Rabbit Polyclonal Antibody for WB and ELISA - Q9NR61

Core Product Specifications and Parameters

Biological Background: DLL4 Function and Localization

• Delta-like protein 4 (DLL4), also known as Drosophila Delta homolog 4, is a transmembrane ligand in the Notch signaling pathway that activates NOTCH1 and NOTCH4 receptors to regulate cell fate decisions. Related references: PMID:11134954
• DLL4 plays a critical role in angiogenesis by negatively regulating endothelial cell proliferation, migration, and angiogenic sprouting. Related references: PMID:20616313
• The protein is essential for retinal progenitor cell proliferation and proper generation of retinal cell types, including suppression of rod fates in late progenitors (By similarity).
• During spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate specification. Related references: PMID:17728344
• DLL4 is a single-pass type I membrane protein localized at the cell membrane, featuring an extracellular region with multiple EGF-like domains, disulfide bonds, and glycosylation sites characteristic of Notch ligands.
• It is predominantly expressed in vascular endothelium, consistent with its key role in vascular development and angiogenesis.
• Post-translational modifications, including glycosylation and proteolytic processing, are critical for DLL4 function as a signaling ligand.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band at ~75 kDa. Use positive controls such as HUVEC or other vascular endothelial cell lysates known to express DLL4.
• The immunogen covers amino acids 317–530 of the extracellular domain; ensure thorough protein extraction and denaturation for membrane protein detection.
• Validate cross-reactivity in mouse or rat samples by aligning the epitope region to confirm sequence conservation; adjust sample preparation as needed.
• For ELISA, optimize coating antigen concentration and use cell lysates or recombinant DLL4 as standards; consider testing multiple detection conditions for best signal-to-noise ratio.
• Include appropriate negative controls (e.g., DLL4-deficient lysates) to confirm antibody specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-DLL4 antibody supports angiogenesis, Notch signaling, and developmental biology research, a key focus area for our customers. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare-target raw materials to streamline your assay development.