Anti-DLL4 Rabbit Monoclonal Antibody for WB, IP, FC, ELISA - Q9NR61

Core Product Specifications and Parameters

Biological Background: DLL4 Function and Localization

• DLL4 (Delta-like protein 4) is a member of the Delta/Serrate/Lag-2 (DSL) family of Notch ligands, functioning as a transmembrane ligand for Notch receptors.
• It activates NOTCH1 and NOTCH4, playing a critical role in the Notch signaling pathway. Related references: PMID:11134954
• DLL4 is a key negative regulator of angiogenesis; it inhibits endothelial cell proliferation, migration, and angiogenic sprouting. Related references: PMID:20616313
• During neurogenesis, DLL4 modulates cell fate decisions; for instance, it inhibits V2a interneuron differentiation in the developing spinal cord. Related references: PMID:17728344
• DLL4 is localized to the cell membrane, consistent with its role as a transmembrane signaling molecule.
• It is predominantly expressed in vascular endothelium, underscoring its angiogenic functions.
• The protein contains multiple EGF-like repeats and a transmembrane domain, and is known to be glycosylated and contain disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 27-524) encompasses the entire extracellular domain of DLL4, potentially allowing recognition of the native, cell-surface protein as well as soluble fragments.
• For Western blotting, a band corresponding to ~75 kDa is expected under reducing conditions; testing both reducing and non-reducing conditions may provide additional information on disulfide-linked complexes.
• For flow cytometry, verify that the antibody recognizes the extracellular epitope in live, unfixed cells to ensure native conformation binding.
• For immunoprecipitation, use standard protocols with appropriate negative controls to confirm specificity.
• For ELISA, pair with a suitable detection antibody; consider checking compatibility with recombinant DLL4 protein.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw material sourcing partner, CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of validated antibodies, including high-quality monoclonal antibodies like this anti-DLL4 clone for applications such as WB, IP, FC, and ELISA. Whether you are investigating Notch signaling in angiogenesis, developing research tools for vascular biology, or require bulk quantities of ancillary reagents, CamelBio streamlines procurement and provides expert technical consultation to accelerate your project from concept to clinic.