Anti-DLL3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NYJ7

Core Product Specifications and Parameters

Biological Background: DLL3 Function and Localization

• DLL3, also known as Drosophila Delta homolog 3, is a member of the Delta/Serrate/Lag-2 (DSL) ligand family that modulates the Notch signaling pathway.
• It functions primarily as an inhibitor of neurogenesis, diverting neuronal precursors along specific differentiation pathways.
• During early embryonic development, DLL3 is essential for the formation of somite boundaries and proper segmentation of the paraxial mesoderm.
• The protein is a single-pass type I transmembrane protein localized to the plasma membrane.
• Its extracellular region contains multiple EGF-like repeats and a disulfide bond, characteristic of its ligand-receptor interactions.
• Post-translational modifications include Ubl conjugation (e.g., ubiquitination) and alternative splicing, which generate multiple isoforms.
• UniProtKB keywords indicate association with disease variants and dwarfism, aligning with its critical developmental role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 520-618), which may recognize both full-length and alternatively spliced isoforms; validate in your specific sample.
• For Western blotting, a band at approximately 65 kDa is expected under reducing conditions; consider using a positive control lysate from human, mouse, or rat tissues known to express DLL3.
• In immunohistochemistry (paraffin), membrane staining should be observed; optimize antigen retrieval and antibody dilution to minimize background.
• For ELISA applications, the polyclonal nature allows flexibility as capture or detection reagent; test cross-reactivity with homologous proteins if used in multiplex assays.
• Given cross-reactivity with mouse and rat, this antibody can be applied to commonly used model organisms; always include species-appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials. This DLL3 polyclonal antibody supports developmental biology and Notch signaling research, and our team can assist with sourcing validated antibody pairs, bulk ancillary reagents, and rare-target proteins for your entire workflow—from concept to clinic.