Anti-DLK1 Rabbit Polyclonal Antibody for WB, ELISA - P80370

Core Product Specifications and Parameters

Biological Background: DLK1 Function and Localization

• Protein delta homolog 1 (DLK1), also known as pG2, is encoded by the DLK1 gene.
• It may play a role in neuroendocrine differentiation.
• The protein localizes to the plasma membrane and cytoplasm.
• DLK1 is expressed in specific fetal and adult tissues, including placental stromal cells, yolk sac, fetal liver, adrenal cortex, pancreatic islets, and certain neuroendocrine tumors.
• Structurally, DLK1 is a transmembrane protein with an N-terminal signal peptide, six EGF-like repeats, multiple disulfide bonds, and glycosylation sites.
• Alternative splicing of the DLK1 gene generates several isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 83-383), including EGF-like domains; consider testing both reducing and non-reducing SDS-PAGE to account for disulfide bonds.
• Cross-reactivity with mouse suggests the antibody may be suitable for rodent model studies; validate in the appropriate sample system.
• For ELISA, optimize antigen coating conditions and antibody dilution to ensure specificity and sensitivity.
• Perform initial validation using positive control tissues/cells known to express DLK1, such as neuroendocrine tumor samples or pancreatic islets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, from concept to clinic. This DLK1 antibody supports research into neuroendocrine differentiation, and we can supply validated antibody pairs, monoclonal alternatives, and bulk reagents. For rare targets like DLK1, CamelBio provides reliable sourcing to accelerate your diagnostic development.