Anti-DLK1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P80370

Core Product Specifications and Parameters

Biological Background: DLK1 Function and Localization

• Protein delta homolog 1 (DLK1), also known as pG2 or PREF1, is a transmembrane protein containing multiple EGF-like repeats, a signal peptide, and a single transmembrane helix.
• It is expressed in stromal cells of placental villi, yolk sac, fetal liver, adrenal cortex, pancreas, and in beta cells of the islets of Langerhans; also detected in some neuroendocrine lung tumor tissues.
• DLK1 may play a role in neuroendocrine differentiation.
• The protein undergoes alternative splicing, generating multiple isoforms, and is post-translationally modified by glycosylation and disulfide bond formation.
• Subcellular localization includes the cell membrane and cytoplasm.
• The protein is also known by aliases such as Delta1 and FA1, reflecting its identification in various developmental and tumor contexts.
• 3D structural data and direct protein sequencing are available, supporting its classification as a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen (aa 24–303) covers most of the extracellular region, including EGF-like domains, which may facilitate detection of both membrane-bound and shed forms of DLK1.
• For Western blot, consider using both reducing and non-reducing conditions to account for the disulfide-stabilized EGF-like repeats; the predicted molecular weight is ~41 kDa, but glycosylation may shift migration.
• In IHC-P, validate in known positive tissues such as placenta or pancreas, and include neuroendocrine tumor samples if relevant; antigen retrieval conditions should be optimized.
• For IF/ICC, permeabilization is recommended to detect cytoplasmic localization; confirm specificity using knockout or knockdown cell lines where possible.
• Cross-reactivity with mouse and rat is reported; verify performance in the specific species and sample system of interest.
• As a polyclonal antibody, it may recognize multiple isoforms and degradation products; use appropriate controls (e.g., peptide blocking, isotype controls) to confirm target bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies focused on DLK1 and its roles in neuroendocrine biology and development, CamelBio offers sourcing support for rare target raw materials and customized procurement solutions, ensuring a reliable supply chain from concept to clinic.