Anti-DLGAP2 Polyclonal Antibody for WB, ELISA - Q9P1A6

Core Product Specifications and Parameters

Biological Background: DLGAP2 Function and Localization

• DLGAP2 (Disks large-associated protein 2), also known as SAPAP2, is a synaptic scaffolding protein that binds PSD-95/SAP90 and may organize postsynaptic signaling complexes.
• It may function as an adapter linking ion channels to the subsynaptic cytoskeleton, contributing to the molecular organization of excitatory synapses.
• DLGAP2 belongs to the SAPAP gene family, which includes DLGAP1–4, sharing conserved domains involved in protein-protein interactions at the postsynaptic density.
• The protein is localized to the cell membrane, specifically enriched at postsynaptic densities in neuronal synapses, highlighting its role in synaptic structure.
• Expression is detected in the brain and kidney, suggesting tissue-specific functions beyond the central nervous system.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword), and alternative splicing generates multiple isoforms (Alternative splicing keyword) that may diversify its function.
• According to UniProt keywords, DLGAP2 is a membrane-associated phosphoprotein identified via proteomics (Proteomics identification) and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 550–800 within the central region of human DLGAP2. This region may include conserved domains; verify isoform specificity if targeting particular splice variants.
• For Western blot (WB), the predicted molecular weight is approximately 118 kDa. Use appropriate gel percentage and transfer conditions to resolve high-molecular-weight proteins. Employ a validated loading control.
• For ELISA, consider using the antibody to detect recombinant or endogenous DLGAP2 in appropriate sample matrices. Validate cross-reactivity with related family members if necessary.
• Because expression is reported in brain and kidney, these tissues may serve as positive controls. Perform antigen retrieval or enrichment steps if needed for tissue lysates.
• The antibody's cross-reactivity is specified as human; evaluate reactivity in other species empirically if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents to support diagnostic development from discovery to clinical application. With a focus on neuroscience and signaling targets like DLGAP2, CamelBio streamlines your access to high-quality materials, including rare-target sourcing, enabling efficient assay development and scaling.