Anti-DLDH/DLD Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P09622

Core Product Specifications and Parameters

Biological Background: DLDH/DLD Function and Localization

• DLD functions as a critical E3 component in the pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, and branched-chain amino acid dehydrogenase complexes, catalyzing reoxidation of the dihydrolipoyl moiety on E2 with NAD+ as the electron acceptor. Related references: PMID:16442803, PMID:16770810
• It is also an essential component of the glycine cleavage system, linking it to glycine metabolism. Related references: PMID:15712224
• A nuclear fraction of the alpha-ketoglutarate dehydrogenase complex, containing DLD, associates with KAT2A on chromatin to supply succinyl-CoA for histone succinylation. Related references: PMID:29211711
• In its monomeric form, DLD may exhibit a moonlighting serine protease activity. Related references: PMID:17404228
• The protein is involved in sperm capacitation and the acrosome reaction.
• DLD resides predominantly in the mitochondrial matrix but also localizes to the nucleus, cell projections, cilia, flagella, and acrosomal vesicles.
• Post-translational modifications include acetylation, phosphorylation, and disulfide bond formation; the enzyme requires FAD as a cofactor.
• Alternative splicing generates multiple isoforms, and mutations in DLD are linked to metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fragment spanning amino acids 36–300 of human DLD, which may predominantly target the N-terminal domain. Consider this when interpreting band patterns or cross-reactivity.
• For Western blot, the expected molecular weight is ~54 kDa. Validate band specificity in the relevant sample system, as post-translational modifications or isoforms may produce additional bands.
• In IHC-P, optimize antigen retrieval conditions and antibody dilution. Endogenous peroxidase and biotin may need to be blocked depending on the detection system.
• ELISA performance can be influenced by coating antigen purity and format. Titrate the antibody in the appropriate assay buffer to establish working concentrations.
• This antibody shows cross-reactivity with mouse, making it suitable for studies in mouse models. Species cross-reactivity beyond human and mouse has not been specified; independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-DLDH/DLD antibody serves metabolic and mitochondrial disease research, and we offer a full portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Leverage our integrated supply chain to streamline your assay development.