Anti-DLDH/DLD Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09622

Core Product Specifications and Parameters

Biological Background: DLDH/DLD Function and Localization

• DLDH/DLD (dihydrolipoyl dehydrogenase) is a flavoprotein oxidoreductase that uses FAD and NAD as cofactors, existing as a homodimer. It is encoded by the DLD gene, with alternative splicing producing multiple isoforms.
• As the E3 component of the pyruvate, α-ketoglutarate, and branched-chain α-keto acid dehydrogenase complexes, DLDH catalyzes the reoxidation of dihydrolipoamide with NAD+ as electron acceptor, essential for mitochondrial energy metabolism. Related references: PMID:16442803, PMID:16770810, PMID:20160912
• It is also a component of the glycine cleavage system (GCS), participating in glycine degradation and one-carbon metabolism, with the designation GCSL. Related references: PMID:15712224
• In the nucleus, DLDH associates with histone succinyltransferase KAT2A, supplying succinyl-CoA for histone lysine succinylation, linking metabolism to epigenetic regulation. Related references: PMID:29211711
• Monomeric DLDH may function as a moonlighting serine protease, as suggested by cleavage activity independent of its dehydrogenase role. Related references: PMID:17404228
• Subcellular fractionation and immunolocalization reveal DLDH in the mitochondrial matrix, nucleus, and also in cell projections (cilia, flagella) and cytoplasmic vesicles (acrosome), supporting roles in sperm capacitation and acrosome reaction (by similarity).
• Post‑translational modifications include acetylation, phosphorylation, and disulfide bond formation, which may regulate its activity and localization; mutations in DLD cause dihydrolipoamide dehydrogenase deficiency (E3 deficiency).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 401–500 of human DLDH/DLD, a region likely conserved among mammals, consistent with the observed cross‑reactivity to mouse and rat.
• For western blotting (WB), the predicted molecular weight is ~54 kDa (precursor); using reducing conditions helps disrupt disulfide bonds. Include appropriate cellular fractionation controls when assessing mitochondrial versus nuclear localization.
• In immunohistochemistry (IHC‑P) and immunocytochemistry (IF/ICC), validate the antibody in tissue/cell types known to express DLDH, and consider antigen retrieval methods as staining may vary.
• For ELISA applications, confirm binding to recombinant or native DLDH protein under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop provider of IVD raw materials, serving diagnostic manufacturers, labs, and research institutes. This anti‑DLDH/DLD antibody supports investigations into mitochondrial metabolism, glycine cleavage, and histone succinylation. We supply validated antibody pairs, optimized recombinant monoclonals, bulk ancillary reagents, and expert sourcing for rare‑target raw materials to accelerate your assay development from concept to clinic.