Anti-DLD/DLDH Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09622

Core Product Specifications and Parameters

Biological Background: DLD/DLDH Function and Localization

• DLD/DLDH is a mitochondrial enzyme that functions as the E3 component of the pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and branched-chain α-ketoacid dehydrogenase complexes, and is also a component of the glycine cleavage system. It catalyzes the reoxidation of dihydrolipoamide using NAD+ as an electron acceptor (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:20160912). Related references: PMID:15712224, PMID:16442803, PMID:16770810, PMID:20160912
• A fraction of the α-ketoglutarate dehydrogenase complex localizes to the nucleus, where DLD/DLDH provides succinyl-CoA to the histone succinyltransferase KAT2A, contributing to lysine succinylation of histones on chromatin (PubMed:29211711). Related references: PMID:29211711
• In its monomeric form, DLD/DLDH may exhibit an additional moonlighting function as a serine protease (PubMed:17404228). Related references: PMID:17404228
• The protein is involved in the hyperactivation of spermatozoa during capacitation and is implicated in the acrosome reaction, highlighting a role in reproduction (By similarity).
• DLD/DLDH is primarily localized to the mitochondrial matrix, but is also found in the nucleus, secretory vesicle acrosome, and flagellum/cilium, reflecting its multifunctional roles across compartments.
• Key structural features include a FAD cofactor, a redox-active disulfide center, and a mitochondrial transit peptide. The protein is subject to acetylation, phosphorylation, and alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (amino acids 36–300) that is common to all known human DLD isoforms, suggesting broad isoform reactivity; however, validate for your specific isoform of interest.
• For western blot (WB), the expected molecular weight is approximately 54 kDa; note that post-translational modifications such as acetylation or phosphorylation may cause minor band shifts.
• For IHC-P and IF/ICC, appropriate antigen retrieval and permeabilization steps should be optimized; consider the dual mitochondrial and nuclear localization when selecting fixation and detection conditions.
• The antibody cross-reacts with mouse DLD, enabling its use in mouse models; always validate the antibody in the relevant tissue or cell type before assay implementation.
• ELISA protocols can be developed for measuring DLD/DLDH levels in serum, plasma, or tissue lysates; use the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, providing this anti-DLD/DLDH rabbit polyclonal antibody along with complementary antigens and ancillary reagents for mitochondrial metabolism and epigenetics research. Our supply network offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk raw materials to streamline your assay development from concept to clinical application. Let CamelBio simplify your procurement of rare-target reagents and ensure seamless access to high-quality IVD components.