Anti-DLC1 Polyclonal Antibody for WB and ELISA - Q96QB1

Core Product Specifications and Parameters

Biological Background: DLC1 Function and Localization

• DLC1 is a Rho GTPase-activating protein (RhoGAP) that specifically inactivates the small GTPases RHOA, RHOB, RHOC, and CDC42, thereby terminating their downstream signaling cascades.
• It plays a critical role in cytoskeletal reorganization, cell migration, and proliferation, and is recognized as a tumor suppressor in multiple cancer types.
• In addition to its RhoGAP activity, DLC1 functions as an activator of phospholipase PLCD1.
• During growth factor-induced epithelial cell migration, DLC1 forms complexes with TNS3 and PTEN; upon stimulation, phosphorylation triggers a switch where PTEN binds DLC1 and TNS3 binds p85, leading to RHOA activation at the cell posterior and RAC1 activation at the leading edge. Related references: PMID:26166433
• DLC1 localizes to the cytoplasm, focal adhesions, and the cell membrane, consistent with its roles in cell adhesion and migration.
• The protein is most highly expressed in the spleen, with lower levels in prostate, testis, ovary, small intestine, and colon; it is absent from the thymus.
• DLC1 contains a START (StAR-related lipid transfer) domain, reflected in its alternative name STARD12, which may mediate lipid or sterol interactions.
• The gene undergoes alternative splicing and uses alternative promoters, generating multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 204–463, a region upstream of the characteristic RhoGAP domain; this design may provide specificity for DLC1 isoforms without interfering with GAP activity detection.
• For Western blotting, this antibody detects a band around the predicted molecular weight of 171 kDa; consider including positive controls from human, mouse, or rat cell lysates known to express DLC1 (e.g., spleen-derived lines) given the reported cross-reactivity.
• In ELISA applications, optimize antigen coating conditions using recombinant full-length DLC1 or the immunogen fragment and verify linearity of detection with serial dilutions of standard protein.
• Since DLC1 functions in focal adhesion and migration, fixation and permeabilization conditions for immunocytochemistry should be carefully optimized to preserve native localization; note that this antibody has not been validated for ICC/IHC in the product specifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering high-quality antibodies like this anti-DLC1 polyclonal antibody to support tumor biology and cell migration research. In addition to validated antibody reagents for WB and ELISA, we provide customized bulk ordering, matched antibody pairs, and sourcing assistance for rare target antigens. Let CamelBio streamline your assay development with reliable, one-stop access to critical immunoreagents.