Anti-DLAT Rabbit Monoclonal Antibody for WB, ELISA - P10515

Core Product Specifications and Parameters

Biological Background: DLAT Function and Localization

• DLAT encodes the dihydrolipoyllysine-residue acetyltransferase component of the pyruvate dehydrogenase complex (PDC-E2). It is also known as the 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC), M2 antigen complex 70 kDa subunit, and PDCE2.
• As part of the pyruvate dehydrogenase (PDH) complex, DLAT catalyzes the transfer of acetyl groups from acetyl-lipoyl moieties to coenzyme A, forming acetyl-CoA. This reaction links cytoplasmic glycolysis to the mitochondrial tricarboxylic acid (TCA) cycle.
• The PDH complex is a large multienzyme assembly containing multiple copies of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3). DLAT functions as the E2 component.
• DLAT localizes to the mitochondrial matrix, where the PDH complex is active.
• The protein undergoes lipoylation and acetylation, post-translational modifications essential for its enzymatic function and regulation.
• DLAT is a transferase that plays a key role in carbohydrate and glucose metabolism, specifically in the TCA cycle.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 548–647 of human DLAT, a C-terminal region. Consider this antigen design when assessing epitope specificity.
• For Western blotting, an expected band is observed at approximately 69 kDa (theoretical molecular weight ~69 kDa). Using mitochondrial-enriched lysates may enhance detection sensitivity.
• Validate reactivity in the relevant species: human, mouse, and rat cell lines or tissue extracts. Include appropriate positive and negative controls.
• For ELISA applications, consider using recombinant DLAT protein or peptide coating; optimize antibody dilution and detection system for your specific assay format.
• Because DLAT is a mitochondrial enzyme, ensure that sample preparation protocols effectively extract mitochondrial proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like anti-DLAT. We provide one-stop access to antibody pairs, ancillary reagents, and rare-target sourcing for mitochondrial metabolism research. Our streamlined supply chain helps accelerate your development from concept to clinic.