Anti-DLAT Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P10515

Core Product Specifications and Parameters

Biological Background: DLAT Function and Localization

• DLAT encodes dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (also known as PDC-E2, DLTA, and the 70 kDa mitochondrial autoantigen of primary biliary cirrhosis (PBC)).
• The enzyme is a core component of the pyruvate dehydrogenase (PDH) complex that catalyzes the conversion of pyruvate to acetyl-CoA, linking glycolysis to the TCA cycle.
• DLAT accepts acetyl groups from the acetyl-lipoyl moiety generated by pyruvate dehydrogenase (E1) and transfers them to coenzyme A, forming acetyl-CoA.
• The protein localizes to the mitochondrial matrix, consistent with its role in mitochondrial energy metabolism.
• It belongs to the 2-oxoacid dehydrogenase complex E2 component family and contains lipoyl-binding domains essential for its acetyltransferase activity.
• Keywords include Acetylation, Acyltransferase, Lipoyl, Mitochondrion, Phosphoprotein, Transit peptide, and Tricarboxylic acid cycle, indicating its regulation and metabolic importance.
• DLAT is recognized as the M2 autoantigen in primary biliary cirrhosis (PBC), an autoimmune liver disease.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 87-270 of human DLAT) corresponds to a central region that may include the lipoyl-binding domain; antibody reactivity to endogenous protein should be validated in the relevant cell or tissue lysates.
• For Western blotting, a band around 69 kDa is expected; perform a positive control using mitochondrial extracts from human, mouse, or rat tissues/cells.
• For IHC-P and IF/ICC, consider antigen retrieval and mitochondrial counterstaining to confirm subcellular localization.
• The polyclonal nature may yield detection of multiple isoforms or degradation products; titrate the antibody for optimal signal-to-noise ratio.
• Due to cross-reactivity with mouse and rat homologs, this antibody may be suitable for interspecies studies, but confirm specificity in each model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This DLAT polyclonal antibody supports research on mitochondrial disorders, metabolic diseases, and autoimmune conditions. In addition to validated antibodies, we offer antibody pairs, bulk reagents, and custom sourcing for rare targets to streamline your assay development from concept to clinic.