Anti-DKK4 (DKK-4) Rabbit Polyclonal Antibody for WB, ELISA - Q9UBT3

Core Product Specifications and Parameters

Biological Background: DKK4 Function and Localization

• Dickkopf-related protein 4 (DKK4) is a secreted glycoprotein belonging to the Dickkopf family of canonical Wnt signaling antagonists.
• It inhibits Wnt/β-catenin signaling by binding to LRP5/6 co-receptors and forming a ternary complex with KREMEN, which promotes LRP5/6 internalization.
• DKK4 plays critical roles in vertebrate embryonic development, locally regulating processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation.
• In adults, DKK4 is implicated in bone homeostasis, cancer progression, and Alzheimer disease, though precise mechanisms remain largely inferred by similarity.
• Subcellular localization: Secreted into the extracellular space.
• Tissue specificity: Expressed in cerebellum, T-cells, esophagus, and lung.
• Post-translational modifications include disulfide bond formation and cleavage on a pair of basic residues, as indicated by direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen spans the mature secreted form (aa 19–224) of human DKK4, so the antibody may recognize both full-length and processed forms; optimize detection conditions accordingly.
• For Western blot, a band near 25 kDa is expected. Confirm specificity with appropriate controls such as siRNA knockdown or recombinant protein competition.
• Cross-reactivity with mouse DKK4 is indicated; validate performance in your specific mouse tissue or cell line.
• For ELISA, consider titration experiments to determine optimal coating and detection antibody concentrations for your assay format.
• Use standard blocking and washing protocols; if background is high, reduce antibody concentration or increase blocking stringency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized polyclonal antibodies like this DKK4 pAb, bulk ancillary reagents, and rare target raw materials. Our integrated sourcing platform supports your Wnt signaling and developmental biology studies from concept to clinic. Contact us for customized procurement solutions and technical guidance.