Anti-DKK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UBP4

Core Product Specifications and Parameters

Biological Background: Dickkopf-related protein 3 Function and Localization

• Dickkopf-related protein 3 (DKK3), encoded by the DKK3 gene (also known as REIC), is a member of the dickkopf family of secreted Wnt antagonists.
• DKK3 antagonizes canonical Wnt signaling by inhibiting the interaction between LRP5/6 co-receptors and Wnt ligands, and also promotes LRP5/6 internalization through complex formation with KREMEN.
• During vertebrate development, DKK3 locally inhibits Wnt-regulated processes including antero-posterior axial patterning, limb development, somitogenesis, and eye formation.
• In adults, DKK3 is implicated in bone formation, bone disease, cancer, and Alzheimer's disease.
• DKK3 is a secreted protein, with a signal peptide, and is post-translationally modified by glycosylation and disulfide bond formation; it also contains a coiled-coil domain.
• The protein shows highest expression in heart, brain, and spinal cord.
• As a reference proteome protein, DKK3 has been identified in proteomics studies and direct protein sequencing.

Experimental Guidance and Technical Tips

• For Western blotting, the protein has a predicted molecular weight of approximately 38 kDa, but glycosylation may cause a shift; validate with appropriate controls.
• In immunofluorescence/immunocytochemistry, as DKK3 is secreted, consider using secretion inhibitors or staining under conditions that retain extracellular matrix-bound protein; validate in relevant cell types.
• When using this antibody for ELISA, the immunogen covers the N-terminal region (aa 22-200), which may recognize DKK3 even in complex with other proteins; perform sandwich ELISA optimization.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for model organism studies; however, confirm sequence homology in the immunogen region before use.
• Tissue specificity data suggest high expression in heart, brain, and spinal cord; these tissues are recommended as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to supply high-quality IVD raw materials, including validated antibody pairs and bulk reagents. For Wnt signaling and cancer research, CamelBio offers this anti-DKK3 polyclonal antibody alongside a wide range of ancillary reagents and custom sourcing services. Our one-stop platform supports your development from concept to clinic with reliable, scalable solutions.